This lab assignment is due by 23:59 on 2024-09-17. If you have any
questions or need clarification, please reach out to me via email or
during office hours. The report will be graded on 20
points based on the following criteria:
Criteria
Points
Introduction
1
Data Collection
2
Methods
4
Results
5
Discussion
5
Conclusion
3
Submission: Please submit your report as a PDF file
on Canvas. Make sure to include your name and your partner’s name at the
beginning of the report. Include any code, plots, or tables as needed to
support your answers. Make sure to answer all questions thoroughly and
provide detailed explanations where necessary. Cite any external sources
used. Submit one single pdf file with all the answers.
Collaboration with your classmates is encouraged,
and you will work in pairs for this lab to complete the report. However,
each student must collect their own data independently. Please list your
partner’s name at the beginning of the report. Only one submission per
group is required. Must include data from both partners in the
report.
Late submissions will be penalized by a 1 point
deduction every hour past the deadline.
Please read the course policy on academic integrity and collaboration
on the course syllabus. If you have any questions about what is
permissible, please ask before submitting your work.
Introduction
In this lab, you’ll explore how your eyes adapt to low light
conditions (dark adaptation) and experience the Purkinje shift—a change
in how your eyes respond to different colors as lighting changes. These
processes are essential for understanding how we see in various
environments.
Background
Understanding how human vision adapts to different lighting
conditions is crucial in designing environments and interfaces that
accommodate various visual scenarios. This lab will explore:
Dark adaptation: How the eye adjusts to low light conditions over
time
The Purkinje shift: Changes in color perception between bright and
dim lighting
Cross-modal illusion induced by asymmetric dark-adaptation
Dark adaptation [1]Gray Scale [1]. The leftmost grid has a
luminance of 255 units, and each subsequent grid decreases by five in
luminance.Color Squares [1]
Objectives
By the end of this lab, you should be able to:
Explain how dark adaptation works.
Observe and describe the Purkinje shift.
Collect, graph, and analyze data related to visual thresholds.
Materials
Printed grayscale chart (24 rectangles of decreasing luminance)
Printed color squares (one red, one blue, iso-radiant)
Stopwatch or timer
Access to a dark room or a space where lighting can be
controlled.
(Optional) A photometer to measure light levels.
Procedure
Part 1: Light Adaptation
Start in a well-lit room (about 750 lux) so your eyes can adjust to
bright light.
Take a look at the grayscale printout and mark which rectangles you
can see against the black background. Write time (in minutes) under the
rectangles you can detect.
Now, compare the red and blue squares. Which one looks brighter to
you? Make a note of it.
Part 2: Dark Adaptation
Turned off the lights, creating a very low light environment (about
0.5 lux).
As soon as the lights go off, mark which part of the grayscale you
can still see (this is your 0-minute mark).
Every two minutes, update your chart by marking the rectangle you
can just barely see. Write the time (e.g., 2, 4, 6 minutes) under the
corresponding rectangle.
After 30 minutes, when your eyes are fully dark-adapted, check the
red and blue squares again. Which one looks brighter now? (Remember, you
wouldn’t see color anymore since your rod cells are now doing most of
the work.)
Part 3: Asymmetric Dark
Adaptation
Close one of your eyes and cover it tightly with your hand.
The lights will be turned back on for two minutes to re-adapt your
open eye to the light.
After two minutes, turn off the lights again (i.e. dark room)
Open each eye separately and see how well you can detect the
grayscale. Mark your results for each eye.
Now, open both eyes and pay attention to any unusual sensations or
differences in vision, especially with the eye that was covered.
Results
You’ll plot your data showing how your ability to detect light
changes over time as your eyes adapt to the dark (Plot individual and
class average dark adaptation curves)
Look for the “rod-cone break” on your graph—this shows when your
vision switches from using cones to rods.
Compare your results with your classmates’. Discuss any differences
and think about factors like your position in the room, individual
differences in dark adaptation, and how quickly your eyes adjust to low
light.
Time (minutes)
Threshold Luminance
0
2
…
30
Discussion
Discuss your findings and how they compare to the expected results
for each of the three objectives.
Discuss what dark adaptation and the Purkinje shift are and why they
matter.
Discuss any two of the following:
How might dark adaptation curves inform the design of environments
that transition from bright to dark areas (e.g., movie theaters,
cockpits)?
Given the Purkinje shift, how should this influence the color
choices for emergency or low-light signage?
How could the cross-modal illusion impact the design of virtual
reality systems or other immersive environments?
What are some real-world applications where understanding these
visual phenomena would be crucial for human factors specialists?
How might these visual adaptations differ among various user
populations (e.g., elderly, color-blind individuals)?
What are some limitations of this lab setup, and how could you
improve the experiment to gather more accurate data?
Conclusion
Include a brief summary of your findings and any insights you gained
from this lab. What did you learn about how your eyes adapt to different
lighting conditions, and how might this knowledge be useful in other
contexts?
References
Wolfe U., Ali N. (2015). Dark Adaptation and Purkinje Shift: A
Laboratory Exercise in Perceptual Neuroscience. Journal of
Undergraduate Neuroscience Education, 13(2):A59-A63.